Date: 2015-04-01
Type of information: Presentation of results at a congress
phase:
Announcement: presentation of results at the 2015 Experimental Biology conference in Boston
Company: Organovo (USA - CA)
Product: three-dimensional kidney tissue
Action
mechanism: The kidney proximal tubule tissue is multi-cellular and fully human, consisting of polarized renal proximal tubular epithelial cells (RPTEC) and a living interstitial layer comprised of renal fibroblasts, and endothelial cells. Immunohistochemical analysis shows clear evidence of intercellular junction formation (E-Cadherin+) between the epithelial cells, extensive formation of microvascular structures, and stable maintenance of the layered architecture for at least two weeks in vitro. The epithelial cells within the multi-layered tubular model express CYP450 mRNAs and possess gamma glutamyl transferase (GGT) activity, both of which are indicators of RPTEC function. Importantly, initial characterization shows that the expression of GGT increases over two weeks post-fabrication, indicating a gain-of-function over time.
Disease:
Therapeutic area: Kidney diseases - Renal diseases
Country:
Trial details:
Latest
news: * On April 1, 2015, Organovo, a three-dimensional biology company focused on delivering breakthrough 3D bioprinting technology, presented data on the company\'s in vitro three-dimensional kidney tissue at the 2015 Experimental Biology conference in Boston, Massachusetts. Rully human kidney proximal tubular tissues that have been generated that are three-dimensional, and consist of multiple tissue-relevant cell types arranged to recapitulate the renal tubular / interstitial interface. The tissues are fabricated using Organovo\'s proprietary NovoGen™ bioprinting platform, and will ultimately join the company\'s exVive3D™ Liver tissues to expand the repertoire of physiologically-relevant tissue systems available for toxicity and efficacy testing as well as disease modeling. Dr. Sharon Presnell, Chief Technology Officer and Executive Vice President of Research and Development, stated, \"Our bioprinted human kidney tissue represents a significant technical advance over the simple monolayer cell line cultures that predominate today. The histologic and functional features of the initial prototypes are compelling, and the in vitro durability of the system will likely enable the assessment of drug effects at chronic, physiologically relevant doses. Furthermore, the cellular complexity of the system will likely support mechanistic investigations into drug responses, including end points that have been difficult or impossible to assess in vitro, including tubular fibrosis and post-injury recovery.\" The kidney proximal tubule tissue is multi-cellular and fully human, consisting of polarized renal proximal tubular epithelial cells (RPTEC) and a living interstitial layer comprised of renal fibroblasts (RF), and endothelial cells (EC). Immunohistochemical analysis shows clear evidence of intercellular junction formation (E-Cadherin+) between the epithelial cells, extensive formation of microvascular structures, and stable maintenance of the layered architecture for at least two weeks in vitro The epithelial cells within the multi-layered tubular model express CYP450 mRNAs and possess gamma glutamyl transferase (GGT) activity, both of which are indicators of RPTEC function. Importantly, our initial characterization shows that the expression of GGT increases over two weeks post-fabrication, indicating a gain-of-function over time.
